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The peroxidase-conjugated goat anti-rabbit (Sigma-Aldrich, A6154) or peroxidase-conjugated goat anti-mouse (Sigma-Aldrich, A4416) second antibodies were diluted 1:10,000 and incubated 1 h at RT

Ratio (in %) of mice with cataracts per total mice in each age group is shown in the graphs (Fig 3A and B)

Declining NAD+ induces a pseudohypoxic state disrupting nuclear-mitochondrial communication PubMed

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The method comprises (1) obtaining first a plurality of selected library members comprising a displayed antibody and an associated polynucleotide encoding said displayed antibody, and obtaining said associated polynucleotide encoding for said displayed antibody and obtaining said associated polynucleotides or copies thereof, wherein said associated polynucleotides comprise a region of substantially identical variable region framework sequence, and (2) introducing said polynucleotides into a suitable host cell and growing the cells under conditions which promote recombination and reductive reassortment resulting in shuffled polynucleotides