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For the latter assay, VEC were rinsed with DPBS and then stained with fluorescent antibodies prior to analysis in an Accuri C6 flow cytometer (BD Biosciences)

Bacteriostatic water is a solution of sterile water and benzyl alcohol

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Through shared precursors, metabolic interactions, and functional collaborations, MLT and intestinal Trp metabolites constitute a classic hostmicrobial co-metabolic network (92), which has also been reported to have certain anti-osteoporosis effects