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Particles in good 2D classes were used for ab initio 3D reconstruction (300/1000 initial/final minibatch size parameters), heterogeneous refinement (default settings), and homogeneous (default settings), non-uniform (initial lowpass resolution set to 20 , with a maximum align resolution of 3 ) and local refinements (activated pose/shift gaussian prior) according to the supplemental workflow scheme (Supplementary Figs

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