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Example 4 Step 1: weighing injection water with the prescription amount of about 30 percent, cooling to 20-30 , and filling high-purity nitrogen into the injection water to replace oxygen in the solution until the oxygen content in the solution is less than 1.5 ppm

Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)

For instance, roGFP2-Orp1, the fusion of roGFP2 to the thiol peroxidase enzyme Orp1 is usually employed as a HO-sensitive probe and can be used to determine how chemicals induce or drugs decrease production of ROS in cells (AN252: Real-time monitoring of genetically encoded redox probes in mammalian cell monolayers)

doi: 10.1111/j.1365-4632.2005.02637.x

K., and S