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TNF- serves as a soluble toxic medium produced by liver Kuff cells (KC), mediating the inflammation, tumor cell proliferation and apoptosis

ScienceDirect , Jianhong, et al

10.1007/s10522-014-9538-8 21 BradshawM

[31] Research has found that heavy metals can markedly alter the function and metabolism of many trace elements, including iron, copper, and selenium

HUVECs grown in the 3D model exhibited not only a markedly elevated level of oxidative stress, expressed by an increased level of malondialdehyde and superoxide dismutase activity inhibition, but also an increased inflammatory response with more secretion of IL-1 and IL-6 in response to TNF- when compared to ECs grown on 2D TCPs