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Leptin concentrations are directly proportional to body fat

10.1007/s10143-017-0827-y 464 Van SanE.DebruyneA

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Anti-FLAG magnetic agarose beads (Pierce) were washed on a magnet with 1 mL of ice-cooled IP buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 ) twice and resuspended to their original volume in IP buffer