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Metformin contributes to the therapeutic effects of acne vulgaris by modifying the gut microbiome

Ashwood et al

For analysis of therapeutic efficacy in non-immunized mice, mice (5 mice/group) were first challenged IN with 100x MLD 50 of influenza virus and then administered (24 h post-infection) a single IN dose of drug (zan-DNP, zanamivir, or PBS, mice were anesthetized with isoflurane before the treatment) followed by a single intravenous (IV) dose of rabbit anti-DNP IgG (Invitrogen, cat