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Briefly, cell pellets from MCF-7 were suspended in cold extraction buffer (10 mM HEPES, pH 8.0, 0.34 M sucrose, 10 mM KCl, 1.5 mM MgCl 2 , 0.1% Triton X-100) and incubated on ice for 30 min

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The goal was to isolate the fat-burning effects of growth hormone without its proliferative side effects