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Additionally, NAC cleaves disulfide bonds in proteins, especially in mucin glycoproteins, thereby reducing mucus viscosity and exerting mucolytic effects ( Beyond these well-established clinical indications, NAC exhibits a wide range of pleiotropic effects, including regulation of mitochondrial function, reduction of inflammation and oxidative stress, modulation of apoptosis, and antimicrobial activity through inhibition of biofilm formation in the lungs ( 4 Effects of the combined application of hyperbaric oxygen therapy and N-acetylcysteine In all studies presented, HBOT was administered using clinically relevant protocols, with pressures ranging from 2 to 2.8 ATA and session durations of 60120 min, applied as acute, single, or intermittent treatments (48)

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Clarifying the causal relevance of these signatures will require longitudinal human studies and targeted epigenetic manipulation in defined neural circuits