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To investigate the potential ligating Cys in GSDME, AlphaFold structure of GSDME (AF-O60443-F1-model_v2) was used as the initial structure in view that the experimental GSDME structure remains still unresolved

Du Vigneaud was awarded the Nobel Prize in Chemistry for his work on the synthesis of essential biological substances, including his research on methionine and homocysteine [5]