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Add 1 mL of 50 mmol/L sodium bicarbonate aqueous solution and vortex to mix for 3 min to terminate the reaction

This washing procedure was repeated six times and the adult worms were transferred to 6-cm RNAi plates containing a bacterial lawn (HT115, empty vector or ash-2 RNAi)

The hydrochloride salt ensures the compound maintains its structural integrity during digestion, allowing for optimal absorption in the small intestine