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Unlike traditional cigarettes, e-cigarettes are loaded with a vaporizable liquid

Thereafter, the wells were washed three times with phosphate-buffered saline (PBS) to remove unattached bacteria and then 200 L of 100% methanol was added to each well to fix the attached cells at room temperature for 20 min

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A ban on smoking in public will contribute to reducing these costs by forcing smokers to quit the habit and preventing tobacco-related diseases among nonsmokers." This section might also refer to the usually overlooked economic burden smokers impose on the public health system