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Furthermore, ZIL enabled preparation of stock solutions of platinating agents, whose anticancer effects are completely abolished by dissolution in DMSO

On an experimental day, cells were washed with Hanks Balanced Salt Solution (HBSS, Gibco/ThermoFisher Scientific) and the respective receptors were labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corporation, Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/ThermoFisher Scientific) (2 h, 37 C, 5% CO 2 )

Acta 1733 , 9099 (2005)

The pathological mechanisms and potential therapeutic drugs for myocardial ischemia reperfusion injury