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Abbreviations GPER: G protein-coupled estrogen receptor ER: Estrogen receptors ER: Estrogen receptors GPR30: G protein-coupled receptor 30 MAPK: Mitogen-activated protein kinase ERK-1/2: Extracellular signal-regulated kinase 1/2 IUPHAR: International Union of Basic and Clinical Pharmacology TM: Transmembrane GPCR: G proteincoupled receptor Epi: epicatechin NO: Nitric oxide PFCs: Perfluorinated compounds Gs: Gs alpha subunit Gq: Gs alpha q AC: Adenylyl cyclase cAMP: Cyclic adenosine monophosphate PKA: Protein kinase A IP3: Inositol trisphosphate DAG: Diacylglycerol PIP2: Phosphatidylinositol-4,5-bisphosphate GDP: Guanosine diphosphate GTP: Guanosine triphosphate PLC: Phospholipase C PKC: Protein kinase C AMPK: AMP-activated protein kinase LKB1: Liver kinase B1 CaMKK: Calcium/Calmodulin-dependent protein kinase beta ACC: Acetyl-CoA carboxylase GLUT4: Glucose transport protein 4 HMGCR: 3-hydroxy-3-methylglutaryl-CoA reductase CPT1: Carnitine palmitoyl transferase 1 PPAR: Peroxisome proliferator-activated receptor alpha FAS: Fatty acid synthase SREBP-1c: Sterol regulatory element-binding protein 1c TNF-: Tumor necrosis factor alpha IL-1: Interleukin-1 beta IL-6: Interleukin-6 NF-B: Nuclear factor kappa-light-chain enhancer of activated B cells PI3K: Phosphoinositide 3-kinase AKT: Protein kinase B IBD: Inflammatory bowel disease PEPCK: Phosphoenolpyruvate carboxykinase G6Pase: Glucose-6-phosphatase DHEA: Dehydroepiandrosterone Nrf2: Nuclear factor erythroid 2-related factor 2 GLUT: Glucose transporter eNOS: Endothelial nitric oxide synthase KLF2: Krppel-like factor 2 ATGL: Adipose triglyceride lipase VDR: Vitamin D receptor TRPV6: Transient receptor potential vanilloid 6 SOD: Superoxide dismutase GPx: Glutathione peroxidase Runx2: Runt-related transcription factor 2 VSMCs: Vascular smooth muscle cells T2D: Type 2 diabetes GLUT2: Glucose transport protein 2 MBD: Metabolic bone disease RANK: Receptor activator of nuclear factor kappa- RANKL: Receptor activator of nuclear factor kappa- ligand OPG: Osteoprotegerin PCOS: Polycystic Ovary Syndrome DHT: Dihydrotestosterone LH: Luteinizing hormone FSH: Follicle-stimulating hormone HPO: Hypothalamicpituitaryovarian CRP: C-reactive protein NAFLD: Nonalcoholic fatty liver disease MS: Metabolic syndrome IRSs: Insulin receptor substrates SHRs: Spontaneously hypertensive rats HO: Hydrogen peroxide CVDs: Cardiovascular Diseases PTC: Papillary thyroid carcinoma TCGA: The Cancer Genome Atlas TSH: Thyroid-stimulating hormone WAT: White adipose tissue UCP1: Uncoupling protein 1 KO: Knockout GLUTs: Glucose transporters HK: Hexokinase HIF-1: Hypoxia-inducible factor 1 alpha LDH: Lactate dehydrogenase CAFs: Cancer-associated fibroblasts BCAAs: Branched-chain amino acids BCKDH: Branched-chain -ketoacid dehydrogenase complex Sirt1: Sirtuin 1 HMGB1: High-mobility group box 1 PD-L1: Programmed death-ligand 1 References Notas G, Kampa M, Castanas E

From this point forward, all washing steps should be performed with the standard Binding/Wash buffer, unless you are working with a membrane protein

10.1097/00004647-199708000-00007 347 ZamaniM.RezaiianF.SaadatiS.NaseriK.Ashtary-LarkyD.YousefiM.et al (2023)

Increased rates of fibromyalgia following cervical spine injury