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Why Storage Rules Exist: The Chemistry Behind Stability Peptide degradation in solution occurs through three primary chemical pathways: hydrolysis (water cleaves the peptide backbone, most rapid at elevated temperature and acidic or alkaline pH), oxidation (methionine, cysteine, and tryptophan side chains react with dissolved oxygen or photocatalytic radicals), and deamidation (asparagine and glutamine residues lose an amide group, producing aspartate or glutamate and altering the peptide's charge and receptor binding)

To examine the depletion of GSH content in EC109DDP cells by CDDP combined HCFe nanozyme, the EC109DDP cells were seeded in 6-well plates at a density of 2 10 5 cells and cultured overnight for adherence

doi: 10.1016/j.ccell.2021.05.002

The connection is not fully understood, but several potential mechanisms exist