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The following day, the sections were washed in Duolink Wash Buffer A (0.01 M Tris-Base, 0.15 M NaCl, 0.05% Tween-20, pH 7.4), followed by incubation in Duolink PLA MINUS (Sigma Aldrich, DUO82004) and PLUS probes (Sigma Aldrich, DUO82002) at 37 C for 1 h

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