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A program without a target cannot be optimized through medicinal chemistry, cannot generate target-engagement biomarkers and cannot be de-risked for off-target liabilities

TNF-, belonging to the Fas family, exhibits a robust interaction with the Fas ligand and triggers caspases, initiating programmed cell death ( Moreover, endothelial and leukocyte cell adhesion molecules like ICAM-1 and VCAM-1 promote the recruitment of leukocytes and immune cells to the site of injury through their interactions with endothelial cells (Frugier et al., 2010)

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5.4 Experimental validation of epigenetic mechanisms Recent technological advances in genome editing and single-cell analysis have provided powerful tools to validate EED-induced epigenetic modifications as causal mechanisms underlying transgenerational reproductive toxicity