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mTORC1-mediated translational elongation limits intestinal tumour initiation and growth

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The kinase reactions were conducted in 50 l kinase reaction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 mM MgCl 2 ) with 0.2 mM ATP for 20 min at 30 C

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