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Simultaneously, studies using molecular biology detection and pharmacological intervention confirmed that heat stress triggers cPLA2 activation and increases the level of its metabolite arachidonic acid to participate in lipid peroxidation in the mouse heatstroke model, further promoting the occurrence of ferroptosis, thereby aggravating the progression of ALI after heat stress [48]

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Winter L, Pawlowsky L, Marcinek A, Brauchle B, Muth A, Kazerani M, Petrera A, Kischel R, Emhardt AJ, Rothenberg-Thurley M, Dufour AM, Spiekermann K, Andreeff M , Daver N, Buecklein VL, and Subklewe M

Culture-Independent Approach Besides that, different approaches have been developed to study the microbiome, including clone libraries, terminal restriction fragment length polymorphism (T-RFLP), quantitative PCR (qPCR), transcriptome microarrays, as well as high throughput sequencing technologies (Illumina sequencing and 454 pyrosequencing)

GSH is an important compound in cells because it is involved in the maintenance of cellular homeostasis, regulation of sulfur transport, conjugation of metabolites, xenobiotic detoxification, antibiotic resistance, enzymatic regulation, and the expression of stress response genes [9]