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Mimicking IVD Structure The IVD is composed of a central NP surrounded by an AF and upper and lower hyaline cartilage endplates

Cell Host Microbe

An electrosprey ionization (ESI) source in negative ion mode was employed, with operational conditions set to 12 L/min N 2 as the drying gas, 200 C drying gas temperature, 3000 V capillary voltage, and 50 psi nebulizer pressure

Ex vivo optical imaging Ex vivo fluorescent measurements were performed immediately after organ collection to determine the accumulation of the dye and organs were then stored at 80 C until further analysis