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A total of 12 overnight fasted rats of either sex were used in this investigation

Subsequently, total RNA was reverse-transcribed into cDNA using HiScript 1st Strand cDNA Synthesis Kit (Vazyme, China) following the manufacturers instructions

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doi: 10.3389/fnut.2022.1007816 Received 31 July 2022 Accepted 12 October 2022 Published 01 November 2022 Volume 9 - 2022 Edited by Yannan Jin, De Montfort University, United Kingdom Reviewed by Mostafa Waly, Sultan Qaboos University, Oman

You can do it once or twice a month, and then some people can hold out six to eight weeks, depending on what youre doing