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Short trial periods on HU therapy ( 2 cm, portal fibrosis and irregular chelation history (class 1 = no risk factor, class 2 = one or two risk factors and class 3 = three risk factors)

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To explore the functional relevance of these DEGs, gene ontology (GO) enrichment analysis was conducted, revealing that the altered genes were primarily involved in immune response regulation, cytokine production, oxidative stress response, and mitochondrial functions