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Ethanol-fixed cells were stained with propidium iodide (PI) at room temperature for 1 h in PBS containing 20 g/ml PI (Sigma-Aldrich, St

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Asmussen E, Hald J, & Larsen V (1948) The pharmacological action of acetaldehyde on the human organism

Bio-Rad was used to transfect the brain slices with a human htt exon-1 expression construct containing a 73 CAG repeat (HttN90Q73) in the gWiz backbone (Genlantis) together with a YFP expression construct to visualize transfected neurons

Specifically, the creators detailed moniliformin and citrinin as the most fundamentally impacted compounds for the citrus extract cycle, likewise affecting glycolysis and energy digestion