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K.StrasserS

A reduction in butyrate-producing bacteria, such as Faecalibacterium prausnitzii and Alistipes shahii, weakens the production of SCFAs, leading to the downregulation of HIF-1 signaling, loss of epithelial hypoxic zones, and ultimately the disruption of barrier integrity [97,98,99]

the methods provided herein can include contacting an organ, tissue, or cell with a solution containing particular concentrations and amounts of certain Methods can include placing an organ in a solution containing one or more of the following: 5 to 40 mM sorbitol, 50 to 120 mM potassium D-gluconate, 10 to 40 mM D-saccharic acid (potassium salt), 15 to 30 mM potassium phosphate monobasic, 3 to 12 mM potassium citrate monobasic, 8 to 12 mM magnesium chloride, 2 to 7 mM D-(+)-fructose, 1 to 6 mM glutathione (reduced), 3 to 7 mM adenosine, 20 to 40 mM sodium hydroxide, 10 to 40 mM potassium hydroxide, 1 to 5 percent hydroxyethylstarch, 140 to 180 mEq/L potassium, 20 to 40 mEq/L sodium, 8 to 12 mEq/L magnesium, the particular components can be included in amounts sufficient to maintain metabolic function and viability of the organs, tissues, and cells due to maintenance not only of plasma membrane integrity, but also nuclear and mitochondrial membrane integrity

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