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An unbalanced caloric and protein intake due to incorrect food consumption, responsible for severe under- or over-weight, leads to alterations of the ovarian function with subsequent increase in the infertility

Ethics declarations Ethics approval All animal procedures were conducted in compliance with ethical guidelines and approved by the Animal Care and Use Committee of Shandong Agricultural University (SDAU-2023-038)

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Scientific reports This study aimed to assess differences in fresh semen quality between mature and senile dogs, focusing on oxidative stress markers, including spermatozoa lipid peroxidation and enzymatic antioxidant activity

A useful tool for imaging thiol redox potential is a redox-sensitive green fluorescent protein (roGFP2) expressed in plants, which allows monitoring the redox status in selected parts via microscopy (Meyer et al