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Briefly, the ELISA plate (Perkin Elmer) was coated with the monoclonal antibody against the -subunit of the insulin receptor (Invitrogen, AHR0231)

doi: 10.2165/00003088-199631010-00005

In two rat nerve injury models, nicotine exposure not only reduced pain behaviors but also inhibited the nerve regeneration process in an 7 nicotinic acetylcholine receptor-dependent manner