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A heatmap was made by using the metric fragments per kilobase of feature per million mapped reads (FPKM) value of each transcript, and genes associated with the selected pathways were prominent on the heatmap

To terminate the reactions, an SDS loading buffer was introduced, and subsequent analyses were performed by transferring the samples to PVDF membranes for probing with either an anti-phospho-S32-RIPK1 or an anti-phospho-S166-RIPK1 specific antibody

This distinction is crucial for patients with chronic conditions like multiple sclerosis, where baclofen may provide more effective relief from debilitating spasticity