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Western blot analysis Western blotting was performed as previously described via the following antibodies: FBXO22, BACH1, KLF4, LKB1, SNAL1, PTEN, KDM4A, BAG3, and GAPDH (all from Proteintech, Rosemout, IL)

When the experimental parameters were tuned just right, the hot systems particles almost immediately found their final configuration, cooling exponentially faster than the warm systema situation that Raz, Vucelja, and colleagues had predicted and named the strong Mpemba effect. They reported the results in a 2020 Nature paper and published similar experiments showing the inverse Mpemba effect in PNAS earlier this year

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Keep hydrated (6 glasses of water daily) and take a multivitamin

Vitamin C should be in a separate phase or product