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Wash the collected cells twice by resuspending them in D-PBS without Mg, Ca (Thermo Fisher Scientific 14190136) and spin down

For individuals with diabetes, the risks may be compounded since alcohol can impair self-management behaviors and symptoms of mild intoxication can closely resemble those of hypoglycemia, potentially masking warning signs while metabolizing both substances

These include: Hydroquinone (prescription only in Singapore): A well-studied tyrosinase inhibitor used to reduce focal hyperpigmentation

However, excessive NFE2L2 activation may also promote intracellular cysteine accumulation, which can react with sugars to form adducts that impair cell proliferation 163

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