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The researchers then used the blunt end of a surgical tool to gently compress the thinned area, recreating a concussion

Kinesin, a motor protein responsible for anterograde transport of organelles, vesicles, and protein complexes from the cell body to synaptic terminals, causes transport disruption when mutated

Polymerase chain reaction (PCR) amplification was performed in the presence of 2.25 mM MgCl 2 , 0.08 M of each primer, 0.2 mM dNTP, 1.25 U of Taq polymerase (Roche Diagnostics, Mannheim, Germany) and 5 L of each cDNA to a final volume of 50 L

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Embryos were recovered by standard non-surgical flushing with lactated ringers solution (ICU Medical, San Clemente, CA) supplemented with 1% (v/v) fetal bovine serum on embryonic day 12