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A Spectra Systems FL2000 fluorometer (Spectra Physics, Mountain View, California, USA) was used for detection, with excitation and emission at 394 and 480 nm, respectively

Sequences of qPCR primers can be found in Supplementary Table 1

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10.1056/NEJMoa2031054 26 GarlandP.DurnfordA

Yet, results from another two-year, randomized controlled trial the T reatment O f N onalcoholic fatty liver disease I n C hildren (TONIC) in 173 children (ages, 8-17 years) with MAFLD failed to observe any significant reduction in blood concentrations of alanine and aspartate aminotransferases either with supplemental vitamin E (536 mg/day of RRR --tocopherol) or with metformin (an anti-diabetic drug