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This allows toxic microbial metabolites and other harmful substances to enter systemic circulation, activating the immune system and promoting the release of pro-inflammatory cytokines

In terms of the IKK complex, an innovative small-molecule compound, SR12343, capable of inhibiting NF-B activation was recently identified, which functions by specifically disrupting the IKK-NEMO association

Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)

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Arefhosseini, S