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Observing the model after simulating several initial values and varying limiting agents, we found that pyruvate supply is the limiting substrate of the overall pathway

Cells were pelleted at 1000rpm, resuspended in PBS, and lysed by three freeze-thaw cycles using a dry ice ethanol bath

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The effect of methanethiol and methionine toxicity on the activities of cytochrome c oxidase and enzymes involved in protection from peroxidative damage