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This cycle is repeated 32 times followed by one 5 minute cycle at 70 degree C

doi:10.1007/s12010-011-9436-3 Chen J, Xie L, Cai J, Yang C, Duan X (2013) Enzymatic synthesis of glutathione using engineered Saccharomyces cerevisiae

Beyond these direct effects on redox status, Nrf2 also modulates the expression of genes implicated in cellular defense, thereby inhibiting redox cycle reactions and diminishing GSH consumption (52)