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For gain-of-function studies, M0 macrophages were seeded in a Seahorse XF96 cell culture microplate (50,000 cells per well), transfected with either an empty vector or an HSL-encoding plasmid, and cultured for 24 h

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When pelvic floor muscles become tight they cause painful sex, urinary urgency/frequency and pain

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The problem is that hustle culture, relentless busyness, and the never-ending drive to perform and produce often lead to burnout and perpetuate feelings of exhaustion