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We depleted TP53 using CRISPR-Cas9 in CCLF_PEDS1012T (hereafter PEDS1012T) and CCLF_PEDS_0022T (hereafter PEDS_0022T) as compared to a control gRNA to LacZ (Supplementary Fig

, , This approach is visually really most suitable

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Kumar [79] found that herbicides such as 2,4-Dichlorophenoxy acetic acid and isoproturon progressively reduced carbohydrate and protein content from lower to higher concentrations of the herbicides

Although these findings are primarily derived from murine models, they highlight the dual nature of the gutmammary axis as both a conduit for pathological signals and a mediator of mucosal immune defense, warranting further investigation in dairy cows