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it is a cofactor in DNA synthesis, assists in the metabolism of fatty and amino acids, critical to the central nervous system in the synthesis of the myelin, and the growth of red blood cells in bone marrow

Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

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