In a few plants, the enzyme divinylether synthase converts fatty acid 9-hydroperoxides into divinyl ethers such as colneleic, colnelenic or etheroleic acids as discussed above
Crosslinking experiments pHAT samples (0.5 mg/mL stock), dialysed into (50 mM HEPES-NaOH (pH 7.0), 150 mM NaCl), were incubated with glutaraldehyde at room temperature for 4 h
[DOI] [PubMed] [Google Scholar] Sharma L.K., Lu J., Bai Y
Abbreviations TBK1: TANK-binding kinase 1 NQO1: NAD(P)H: quinone-oxidoreductase-1 IKK: IkappaB kinase NF-B: Nuclear Factor Kappa B RIG-I: Retinoic acid-inducible gene I MDA5: Melanoma differentiation-associated protein 5 cGAS: Cyclic guanosine monophosphateadenosine monophosphate IRF3: Interferon regulatory factor 3 IFN: Interferon IRF7: Interferon regulatory factor 7 ROS: Reactive oxygen species BSL-2: Biosafety Level 2 BSL-3: Biosafety Level 3 VSV-GFP: Green fluorescent protein tagged vesicular stomatitis virus HSV-GFP: Green fluorescent protein tagged herpes simplex virus ELISA: Enzyme-linked immunosorbent assay DMEM: Dulbeccos modified Eagle medium RPMI: Roswell Park Memorial Institute medium PCR: Polymerase chain reaction BMDM: Bone marrow-derived macrophage mGM-CSF: Mouse granulocyte macrophage colony-stimulating factor FBS: Fetal bovine serum MDM: Monocyte-derived macrophages TK-Renilla: Thymidine kinase promoter-renilla RIPA: Radio-immunoprecipitation assay BALF: Bronchoalveolar lavage fluid STAT1: Signal transducer and activator of transcription 1 KD: Kinase domain ULD: Ubiquitin-like domain CC1: Coiled-coiled domain 1 CC2: Coiled-coiled domain 2 PTM: Post-translational modification GSK3: Glycogen synthase kinase-3 beta References Runde AP, Mack R, SJ PB, Zhang J
Msphere 5 (4), 101128 (2020)
