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Description

Apoptotic mechanisms are regulated through a delicate balance of pro- (Bax, caspase-3) and anti-apoptotic (Bcl-2) proteins, governing GC survival

Cells were fixed by vortexing with simultaneous addition of Complete Cell Fixation Solution (Fixation Reagent1, ice-cold 100% methanol, Stanlab, Poland) with DEPC, followed by 15 min incubation on ice and then washed with WRS Buffer (Scale Biosciences) and resuspended in WRS Buffer