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2010), ligaments (Lovric-Bencic et al

It was found that the enzymatic activities of all immobilized samples directly prepared from cell lysates were comparable to those prepared from the conventional immobilization method using purified enzymes

The protein supernatant was collected by centrifugation at 15,000 rpm at 4 for 10 min and the protein concentrations were measured by bicinchoninic acid (BCA) protein assay kit (Beyotime, China)

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