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Total RNA was treated with DNase I (Sigma-Aldrich), quantified, and reverse transcribed into cDNA using the ReverTra Ace- First Strand cDNA Synthesis Kit (TOYOBO, Osaka, Japan)

Importantly, TFEB has no effect on the basal transcriptional levels of downstream target genes but rather regulates their transcriptional levels in response to stimuli

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Different fluorescently labeled cDNA probes were mixed with 30 L of hybridization buffer (3 SSC, 0.2% SDS, 5 Denhardt's solution, and 25% formamide) and applied to the microarray (CapitalBio human mRNA microarray V2.0, CapitalBio, Beijing, China) followed by incubation at 42 C for 16 hours

is derived from Semax ACTH(4-10) the central fragment of adrenocorticotropic hormone, a pituitary peptide