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doi: 10.1016/j.jnutbio.2023.109371 117

A.ShahbazovR.YoshimatsuG.LevyM

Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions, then 1 g of total RNA was prepared for cDNA library construction using a cDNA-PCR Sequencing Kit (SQK-PCS109, Oxford Nanopore Technologies Ltd., Oxford, UK)