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Gene knockdown by RNA silencing siRNAs were transfected into THP-1 macrophages with LipoRNAi MAX transfection reagent (Invitrogen, #13778100)

When the fluorescent secondary antibody method was used, the sections were treated with Alexa 488- or Alexa 555-conjugated anti-mouse/rabbit IgG (1:500 dilution, Molecular Probes, Eugene, OR)

Yes, one-time testing samples can be provided for regulatory and quality approval

(Source 1) Suboptimal and deficient glutathione levels are associated with aging and an increased risk for chronic diseases, including: Mitochondrial dysfunction Neurodegenerative disease, including Alzheimers and Parkinsons disease Cancer Chronic liver disease Metabolic disease, including diabetes and hypertension Cardiovascular disease Pulmonary Infertility Autoimmune diseases, including lupus and multiple sclerosis Mental health disorders Cystic fibrosis (Source 1, 2) A viral illness, including SARS-CoV-2, the virus that causes Covid-19, can also cause glutathione deficiency

Linug Pauling Institute