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Description

Quy cch: 60 vin/ l

Our results, shown on Fig

do not expose to excessive heat

Samples were transferred onto 0.5 m-thick silicon nitride (Si 3 N 4 ) window (Silson), excess buffer wicked away and then the slide was frozen in liquid nitrogen (N 2 )-chilled liquid propane using a KF-80 plunge freezer (Leica Microsystems)

To eliminate technical variability during acquisition, individual samples within one experiment were fixed with Maxpar Fix I Buffer for 10 min at RT, permeabilized with Maxpar Barcode Perm Buffer, and barcoded with the Cell-ID palladium (Pd) barcoding kit (Fluidigm) for 45 min at RT