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The nanoparticles used in this study were designed to be large and rigid (~340 nm in diameter with ~6 nm pore size), which was hypothesized to increase their retention within the TME by preventing leakage

PMID: 31812693 Anal Chem Proximity-Based Phospho-Interactome (Prob-PhI) Characterization Reveals Distinct Signaling Activities of MEK1 and MEK2

The first is conflating injectable and topical dosing conventions applying an injectable milligram-target approach to a topical protocol that should use concentration-based formulation, or vice versa

EMBO J (2012) 31(21):415364

Also, if the injections can be administered as a single shot each time instead of 2 separate shots, it will be less traumatic