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S-palmitoylation at the outer nuclear membrane (SwissKASH assay) HeLa cells were co-transfected as above with plasmids encoding the GFP-fusion, SUN2, and control mRFP/BFP-KASH or mRFP/BFP-zDHHC-KASH at a 1:3:3 plasmid ratio, at a 1:2:2:1 when GCP16/Golga7-Flag, Golga7b-Flag or His 6 -RGS19 was included, or at 1:2:2:1:1 when mRFP-G1 and mRFP-G3 were co-transfected

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Mico JA, Rojas-Corrales MO, Gibert-Rahola J, Parellada M, Moreno D, Fraguas D et al