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The published rodent literature has not reported overt toxicity at the studied amounts, but the available data are not equivalent to a regulated Phase 1/2 safety database

They have realistic expectations about the side effect profile and are willing to tolerate gastrointestinal discomfort in the early weeks

It coincides with other metabolic disorders such as obesity, insulin resistance, diabetes, hypertension, and cardiovascular disease

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Cells were washed twice with CyFACS buffer (PBS with 4% FBS, 0.05% sodium azide), followed by primary antibody staining for 30 mins on ice