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Last, to determine the impact of AKT-dependent regulation of glycolysis on NADPH production we used Fluorescence Lifetime Imaging Microscopy (FLIM) technology to compare NADPH/NADH ratio in AKT-E17K and AKT-WT TrC1 in untreated controls and upon irradiation with 5 Gy with and without additional treatment with the AKT-inhibitor MK2206 ( Figure S3E, F )

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