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For expression in yeast, these sequences were then transferred to pYES-DEST52 vector (Invitrogen, USA) through Gateway cloning technology using LR clonase-II enzyme following the manufacturers instructions

One of NACs potential benefits lies in its ability to create and replenish glutathione, a potent antioxidant in the human body

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Some evidence also exists showing that using aldosterone antagonists such as spironolactone may be beneficial, as high levels of circulating aldosterone have been associated with reduced survival time and aldosterone antagonists reduce proteinuria in man and in a small study in cats (Kai et al, 2022)

The innovative approach in functional bladder disorders: the communication between bladder and brain-gut axis