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A brief (i.e., a few minutes) deprivation of oxygen and glucose, as seen for example in the early stage of acute ischemia or transient ischemic attack, potentiates synaptic transmission at hippocampal CA3-CA1 synapses in an NMDAR-dependent manner and induces ischemic LTP (iLTP) without causing neuronal cell death (Crpel et al., 2015)

These signs overlap with the symptoms of pernicious anemia, because PA is a form of B12 deficiency

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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)